6B6
Catalog Fields
Product Name/ID: 6B6
Available to For-Profits: Yes
Alternate Antibody Name:
Gene Name: cdh3.L
Ab Isotype: MIgG1
Gene Symbol:
Entrez Gene ID: 594865
Clonality: Monoclonal
Immunogen: EC2 extracellular domain - GST fusion protein
Clone:
Immunogen Sequence: Extracellular domain
Myeloma Strain: SP-2/0
Epitope Mapped: No
Antigen Name: cadherin, C- (3)
Epitope Location or Sequence:
Alternate Antigen Name:
Deposit Date: 5/7/1993
Antigen Molecular Weight: 120 kDa
Depositor: Gumbiner, B.M.
Antigen Sequence:
Depositor Institution: University of Virginia
Antigen Species: Xenopus
Depositor Notes: This antibody blocks aggregation of embryonic blastomeres.
Host Species: mouse
Hybridoma Cells Available (Non-Profit): Yes
Confirmed Species Reactivity: Xenopus
Additional Information: RRID: AB_528113
Recommended Applications: FFPE, Function Blocking, Immunofluorescence, Immunoprecipitation, Western Blot
All cell products contain the antimicrobial ProClin. Click here for additional information.
These hybridomas were created by your colleagues. Please acknowledge the hybridoma contributor and the Developmental Studies Hybridoma Bank (DSHB) in the Materials and Methods of your publications. Please email the citation to us.
For your Materials & Methods section:
6B6 was deposited to the DSHB by Gumbiner, B.M. (DSHB Hybridoma Product 6B6)
Storage and Handling Recommendations
Although many cell products are maintained at 4°C for years without loss of activity, shelf-life at 4°C is highly variable. For immediate use, short term storage at 4°C up to two weeks is recommended. For long term storage, divide the solution into volumes of no less than 20 ul for freezing at -20°C or -80°C. The small volume aliquot should provide sufficient reagent for short term use. Freeze-thaw cycles should be avoided. For concentrate or bioreactor products, an equal volume of glycerol, a cryoprotectant, may be added prior to freezing.
Usage Recommendations
The optimal Ig concentration for an application varies by species and antibody affinity. For each product, the antibody titer must be optimized for every application by the end user laboratory. A good starting concentration for immunohistochemistry (IHC), immunofluorescence (IF), and immunocytochemistry (ICC) when using mouse Ig is 2-5 ug/ml. For western blots, the recommended concentration range of mouse Ig 0.2-0.5 ug/ml. In general, rabbit antibodies demonstrate greater affinity and are used at a magnitude lower Ig concentration for initial testing. The recommended concentrations for rabbit Ig are 0.2-0.5 ug/ml (IF, IHC and ICC) and 20-50 ng/ml (WB).
19 References
Initial Publication
Regulation of C-cadherin function during activin induced morphogenesis of Xenopus animal caps.
Gumbiner BM
The Journal of cell biology 126.2 (1994 Jul): 519-27.